By Yonggang Ke, Pengfei Wang
This distinctive quantity provides a complete technical evaluation of DNA nanotechnology with an emphasis on 3D DNA nanostructure layout and purposes. assurance spans from simple layout ideas for DNA and RNA nanostructures to their state-of-the-art purposes in numerous fields, with the ebook divided into simple DNA and RNA nanostructure layout options in addition to purposes using DNA nanostructures, together with yet now not restricted to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the hugely profitable Methods in Molecular Biology sequence, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, and tips about troubleshooting and keeping off identified pitfalls.
Comprehensive and authoritative, 3D DNA Nanostructure: tools and Protocols presents the main up to date instructional variety overviews and technical sort protocols to profit researchers in a wide selection of areas.
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Additional resources for 3D DNA Nanostructure: Methods and Protocols
J Am Chem Soc 116:1661–1669 6. Goodman RP, Berry RM, Turberfield AJ (2004) The single-step synthesis of a DNA tetrahedron. Chem Commun 1372–1373 7. He Y, Ye T, Su M, Zhang C, Ribbe AE, Jiang W, Mao CD (2008) Hierarchical self-assembly of DNA into symmetric supramolecular polyhedra. Nature 452:198–201 8. Zhang C, Su M, He Y, Zhao X, Fang PA, Ribbe AE, Jiang W, Mao CD (2008) Conformational flexibility facilitates selfassembly of complex DNA nanostructures. Proc Natl Acad Sci U S A 105:10665–10669 9.
DNA central strand without a nick leads to a higher assembly yield of DNA nanocages, especially for 4-, 5-, and 6-point star motifs. DNA single strands with a length of 100 nucleotides or more have to be ligated by short DNA component strands. 5. 1× T4 DNA ligase buffer contains 1 mM ATP. 6. The concentration of DNA component strands is important to the yield of DNA ligation. Too high concentration of DNA component strands leads to longer linear structures. 7. Stir the buffer of polyacrylamide gel to make sure that the temperature of the buffer is homogeneous.
Wipe outside of cuvette with lens paper if needed. 4. Pipette 100 μL DNA nanocage-containing sample into the cuvette which is specifically designed for the DLS measurement (10 mm light path facing the incident beam, 2 mm light path facing the detector). Gently tap the cuvette on the bench to remove any bubbles that may appear around the wall of the cuvette. Insert the cuvette in the sample holder on a Malvern Zetasizer Nano-ZS (Malvern Instruments, UK) with a laser wavelength of 633 nm. 5. Setup instrument parameters for DLS analysis.
3D DNA Nanostructure: Methods and Protocols by Yonggang Ke, Pengfei Wang