By David W. Murhammer

ISBN-10: 1493930427

ISBN-13: 9781493930425

ISBN-10: 1493930435

ISBN-13: 9781493930432

The 3rd version of this quantity expands upon the former versions with new and up to date tools and protocols. Chapters contain step by step techniques enthusiastic about quantifying cellphone development, baculovirus an infection and telephone metabolism, the right way to isolate new telephone traces and improve your personal serum-free medium, and regimen upkeep and garage of insect phone traces and baculoviruses, small- and large-scale recombinant protein creation with the BEVS in either insect and mammalian cellphone tradition and in insect larvae, construction and characterization of baculoviruses, eco-friendly fluorescent protein, tubular reactors and RNAi, and baculovirus/insect phone process to review apoptosis and producing envelop-modified baculovirus for gene supply into mammalian cells. Written within the hugely winning Methods in Molecular Biology series structure, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, simply reproducible laboratory protocols, and key tips about troubleshooting and keeping off identified pitfalls.

Authoritative and sensible, Baculovirus and bug mobile Expression Protocols, 3rd Edition goals not to basically reduction the consumer in effectively finishing the initiatives defined, but in addition stimulate the improvement of superior options and new purposes of baculoviruses and bug telephone culture.

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Additional info for Baculovirus and Insect Cell Expression Protocols

Example text

This protein may be a cyclin homolog, involved in host cell cycle arrest during baculovirus infection [36]. An envelope protein of both BV and ODV has also been identified (BV/ODV-E26) and found to be associated with intranuclear microvesicles [37]. A seminal study of protein composition of ODV was conducted by using a combination of techniques [38]. Screening expression libraries with antibodies generated to ODV or BV and mass spectroscopic (MS) analysis of ODV protein extracts identified up to 44 potential components unique to ODV.

J Chem Eng (Japan) 3:18–24 Part II Baculovirus Molecular Biology/Development of Recombinant Baculoviruses Chapter 2 Introduction to Baculovirus Molecular Biology Barbara J. Kelly, Linda A. King, and Robert D. Possee Abstract The development of baculovirus expression vector systems has accompanied a rapid expansion of our knowledge about the genes, their function and regulation in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure and their replication in insect larvae.

While the LD130-encoding baculoviruses do not contain gp64 28 Barbara J. Kelly et al. homologs, those encoding GP64 also contain a version of LD130. The OpMNPV LD130 homolog was found to be a glycosylated protein associated with BV particles and the plasma membrane of cells at similar locations as the GP64 protein [17]. One of the major differences between BV and ODV is that the latter do not contain GP64 or LD130. Instead a virus-encoded envelope protein P74 is associated with the outside of the virus [18, 19].

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Baculovirus and Insect Cell Expression Protocols by David W. Murhammer


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